i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it
Dear Shahnawaz,
You are right; you can dissolve in hot water. See the following properties for Apocynin:
Appearance: Crystalline powder
Physical State: Solid
Solubility: Soluble in ethanol (100 mM), chloroform, ether, hot water, benzene, ethyl acetate, and DMSO (100 mM). Insoluble in Petroleum ether.
Storage: Store at -20° C
Melting Point: 112-115 °C(lit.)
Boiling Point: 263-265 °C at 17 mmHg
Density: 1.16 g/cm3
Refractive Index: n20D 1.54
IC50: NADPH-oxidase: IC50 = 10 µM; Cytochrome b-245 light chain: IC50 = 61000 µM (human); Neutrophil cytosol factor 1: IC50 = 61000 µM (human)
pK Values: pKa: 8.18
Good luck
yes thanks i did same ...
mashaallah, sir
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