I want to dissect corpus cavernosal tissue for organ bath studies in rat. Please send me links where from i can learn them to dissect.
We have published a research paper entitled "Parasympathomimetic Effect of Shilajit Accounts for Relaxation of Rat's corpus cavernosum"; reference: Am J Mens Health March 2013 vol. 7 no. 2 119-127. This paper may be of use for you.
Urgently needed a few protocols. Regarding how to select dose for studying vascular reactivity for a particular drug. How to mention that in objectives? And also share a protocol/procedure that...
08 September 2019 6,301 2 View
Suppose any hypercontractile agent is causing Enos uncoupling and we have noticed contraction in that presence, Now we are incubating the aortic tissue with L-NAME and contractile agent for 1...
01 February 2018 3,382 0 View
If I will increase polarity of solution for protein sample. Will it show increase in OD or decrease in OD. Please give reason.
06 July 2017 3,333 0 View
i am not able to understand bellow mentioned protocol, should i homogenize tissue then to florescence. The intracellular free Ca2+ level in thoracic aorta during contraction wasmeasured based on...
02 March 2016 639 2 View
I am facing problem in Dissolving Quercetin, I have prepared Quercetin 50 mM in 100% Dmso. Then i did serial Dilution to make lower concentration of Quercetin. After Adding some Amount in Micro...
31 December 2015 9,545 4 View
i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it
09 October 2015 8,952 2 View
when we expose our smooth muscle to metal pollutant, it causes scavenging of enzymes like SOD, and GPx , MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury...
06 July 2015 4,033 0 View
I am doing reducing power assay of plant extracts. but after centrifuge of 3000 rpm as per suggested by all protocols that take upper part of layer, but i am not getting any layer formation in...
02 March 2015 2,191 1 View
I have extracted plant leaves in crude form I did not went for any vaporization or deep freezing now. I would like to know antioxident activity and phenolic group concentration, how can I know...
01 February 2015 8,999 6 View
i have plant extract of 22 mg and i want to dissolve it into dmso. can any body tell me procedure for that, i have taken 2 ml of dmso to dissolve it .
01 February 2015 9,801 9 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?
02 August 2024 4,570 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View