How to write a protocol for drug dosage standardisation to study vascular reactivity?

Urgently needed a few protocols. Regarding how to select dose for studying vascular reactivity for a particular drug. How to mention that in objectives? And also share a protocol/procedure that...

08 September 2019 6,154 2 View

What will be over all effect on contraction in presence of L NAME, If we are assuming that contractile agent causes eNOS uncoupling?

Suppose any hypercontractile agent is causing Enos uncoupling and we have noticed contraction in that presence, Now we are incubating the aortic tissue with L-NAME and contractile agent for 1...

01 February 2018 3,228 0 View

What is effect of polarity on OD of proteins?

If I will increase polarity of solution for protein sample. Will it show increase in OD or decrease in OD. Please give reason.

06 July 2017 3,185 0 View

How to determine of intracellular Ca2+ level in rat isolated aorta by a fluorimeter (CAF-110; Jasco, Japan)?

02 March 2016 380 2 View

Does any body knows how to dissect cavernosal tissue from rat?

31 December 2015 5,233 1 View

What is the way to dissolve Quercetin in DMSO. i need final concentration of DMSO 0.01%?

I am facing problem in Dissolving Quercetin, I have prepared Quercetin 50 mM in 100% Dmso. Then i did serial Dilution to make lower concentration of Quercetin. After Adding some Amount in Micro...

31 December 2015 9,266 4 View

What is the procedure to dissolve Apocynin in dmso?

i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it

09 October 2015 8,765 2 View

MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury metals a reversible process or irreversible process ?

when we expose our smooth muscle to metal pollutant, it causes scavenging of enzymes like SOD, and GPx , MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury...

06 July 2015 3,878 0 View

How can I know concentration plant extract with out solidfying the extract for quantifyinf phenolic groups?

I have extracted plant leaves in crude form I did not went for any vaporization or deep freezing now. I would like to know antioxident activity and phenolic group concentration, how can I know...

01 February 2015 8,853 6 View

What is procedure for dissolving 22 mg in dmso? can we use plant extract dissolved in dmso for antioxident study. or dpph assay

01 February 2015 9,618 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View