Shahnawaz Ahmad Wani

13 Questions 23 Answers 0 Followers

Questions related from Shahnawaz Ahmad Wani

How to write a protocol for drug dosage standardisation to study vascular reactivity?

Urgently needed a few protocols. Regarding how to select dose for studying vascular reactivity for a particular drug. How to mention that in objectives? And also share a protocol/procedure that...

09 September 2019 6,245 2 View

What will be over all effect on contraction in presence of L NAME, If we are assuming that contractile agent causes eNOS uncoupling?

Suppose any hypercontractile agent is causing Enos uncoupling and we have noticed contraction in that presence, Now we are incubating the aortic tissue with L-NAME and contractile agent for 1...

02 February 2018 3,317 0 View

What is effect of polarity on OD of proteins?

If I will increase polarity of solution for protein sample. Will it show increase in OD or decrease in OD. Please give reason.

07 July 2017 3,275 0 View

How to determine of intracellular Ca2+ level in rat isolated aorta by a fluorimeter (CAF-110; Jasco, Japan)?

i am not able to understand bellow mentioned protocol, should i homogenize tissue then to florescence. The intracellular free Ca2+ level in thoracic aorta during contraction wasmeasured based on...

03 March 2016 492 2 View

Does any body knows how to dissect cavernosal tissue from rat?

I want to dissect corpus cavernosal tissue for organ bath studies in rat. Please send me links where from i can learn them to dissect.

01 January 2016 5,321 1 View

What is the way to dissolve Quercetin in DMSO. i need final concentration of DMSO 0.01%?

I am facing problem in Dissolving Quercetin, I have prepared Quercetin 50 mM in 100% Dmso. Then i did serial Dilution to make lower concentration of Quercetin. After Adding some Amount in Micro...

01 January 2016 9,439 4 View

What is the procedure to dissolve Apocynin in dmso?

i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it

10 October 2015 8,877 2 View

MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury metals a reversible process or irreversible process ?

when we expose our smooth muscle to metal pollutant, it causes scavenging of enzymes like SOD, and GPx , MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury...

07 July 2015 3,959 0 View

Is there any proper layer formation in reducing power assay?

I am doing reducing power assay of plant extracts. but after centrifuge of 3000 rpm as per suggested by all protocols that take upper part of layer, but i am not getting any layer formation in...

03 March 2015 2,122 1 View

How can I know concentration plant extract with out solidfying the extract for quantifyinf phenolic groups?

I have extracted plant leaves in crude form I did not went for any vaporization or deep freezing now. I would like to know antioxident activity and phenolic group concentration, how can I know...

02 February 2015 8,940 6 View

What is procedure for dissolving 22 mg in dmso? can we use plant extract dissolved in dmso for antioxident study. or dpph assay

i have plant extract of 22 mg and i want to dissolve it into dmso. can any body tell me procedure for that, i have taken 2 ml of dmso to dissolve it .

02 February 2015 9,711 9 View

What can we use instead of rotavapor to concentrate plant extraxt?

Extaction of plants , study of antioxident activity

01 January 2015 4,730 18 View

Will the Indomethacin treated aortic tissue show fluorescence when incubated with DHE for 30 minutes?

I want to know about Indomethacin and role in the generation of reactive oxygen species and relaxation. Will the indomethacin treated aortic tissue show Fluorescence in presence of DHE dye?

01 January 1970 9,350 0 View