If I will increase polarity of solution for protein sample. Will it show increase in OD or decrease in OD.
Please give reason.
Urgently needed a few protocols. Regarding how to select dose for studying vascular reactivity for a particular drug. How to mention that in objectives? And also share a protocol/procedure that...
08 September 2019 6,301 2 View
Suppose any hypercontractile agent is causing Enos uncoupling and we have noticed contraction in that presence, Now we are incubating the aortic tissue with L-NAME and contractile agent for 1...
01 February 2018 3,382 0 View
i am not able to understand bellow mentioned protocol, should i homogenize tissue then to florescence. The intracellular free Ca2+ level in thoracic aorta during contraction wasmeasured based on...
02 March 2016 639 2 View
I want to dissect corpus cavernosal tissue for organ bath studies in rat. Please send me links where from i can learn them to dissect.
31 December 2015 5,396 1 View
I am facing problem in Dissolving Quercetin, I have prepared Quercetin 50 mM in 100% Dmso. Then i did serial Dilution to make lower concentration of Quercetin. After Adding some Amount in Micro...
31 December 2015 9,545 4 View
i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it
09 October 2015 8,952 2 View
when we expose our smooth muscle to metal pollutant, it causes scavenging of enzymes like SOD, and GPx , MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury...
06 July 2015 4,033 0 View
I am doing reducing power assay of plant extracts. but after centrifuge of 3000 rpm as per suggested by all protocols that take upper part of layer, but i am not getting any layer formation in...
02 March 2015 2,191 1 View
I have extracted plant leaves in crude form I did not went for any vaporization or deep freezing now. I would like to know antioxident activity and phenolic group concentration, how can I know...
01 February 2015 8,999 6 View
i have plant extract of 22 mg and i want to dissolve it into dmso. can any body tell me procedure for that, i have taken 2 ml of dmso to dissolve it .
01 February 2015 9,801 9 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...
03 August 2024 3,927 2 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
I am new to gaussian 16 software and while doing non linear optical properties calculation hyperpolarizabiltiy polarizability dipole moment calculation I found different gaussian input keywords...
08 July 2024 7,551 1 View