How to write a protocol for drug dosage standardisation to study vascular reactivity?

Urgently needed a few protocols. Regarding how to select dose for studying vascular reactivity for a particular drug. How to mention that in objectives? And also share a protocol/procedure that...

08 September 2019 6,154 2 View

What will be over all effect on contraction in presence of L NAME, If we are assuming that contractile agent causes eNOS uncoupling?

Suppose any hypercontractile agent is causing Enos uncoupling and we have noticed contraction in that presence, Now we are incubating the aortic tissue with L-NAME and contractile agent for 1...

01 February 2018 3,228 0 View

How to determine of intracellular Ca2+ level in rat isolated aorta by a fluorimeter (CAF-110; Jasco, Japan)?

02 March 2016 380 2 View

Does any body knows how to dissect cavernosal tissue from rat?

31 December 2015 5,233 1 View

What is the way to dissolve Quercetin in DMSO. i need final concentration of DMSO 0.01%?

I am facing problem in Dissolving Quercetin, I have prepared Quercetin 50 mM in 100% Dmso. Then i did serial Dilution to make lower concentration of Quercetin. After Adding some Amount in Micro...

31 December 2015 9,266 4 View

What is the procedure to dissolve Apocynin in dmso?

i want to study muscle contraction mechanism, for that i have to take 100uM concentration . but it is insoluble in water. can i use hot water to disolve it

09 October 2015 8,765 2 View

MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury metals a reversible process or irreversible process ?

when we expose our smooth muscle to metal pollutant, it causes scavenging of enzymes like SOD, and GPx , MY question is whether this metal enzyme complex binding in case of arsenic and/ Mercury...

06 July 2015 3,878 0 View

Is there any proper layer formation in reducing power assay?

02 March 2015 2,026 1 View

How can I know concentration plant extract with out solidfying the extract for quantifyinf phenolic groups?

I have extracted plant leaves in crude form I did not went for any vaporization or deep freezing now. I would like to know antioxident activity and phenolic group concentration, how can I know...

01 February 2015 8,853 6 View

What is procedure for dissolving 22 mg in dmso? can we use plant extract dissolved in dmso for antioxident study. or dpph assay

01 February 2015 9,618 9 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View