Hey Guys,
I am about to set up a drug release experiment and it requires the pH of buffer to be tumorigenic to mimic tumors. I went through literature and it tells that people have used pH ranging 5-6.8. This got me confused a bit as in which buffer I need to make. So, could you please tell me what exactly is the pH of tumor cells and what should be used for drug release experiment?
Thanks.
The extracellular matrix of tumors in vivo is acid (pHe less than 7) while the intracellular pH (pHi) is alkaline (usually above 7). So that this basic concepts of in vivo requirements should be respected in vitro. The researcher has little to do about pHi, but the media in which he is keeping the cells should consider that cancer milieu is hypoxic, low in nutrients and acid.
For further reading I would suggest
https://www.researchgate.net/publication/309225661_Cancer_Fundamentals_behind_pH_targeting_and_the_double-edged_approach
Article Cancer: Fundamentals behind pH targeting and the double-edged approach
It is depending on a lot of factors and it also amazingly varies within various areas of a same tumor.
See the attached articles,
Robert
Hello,
please find another paper : depending on what you want to do and what type of drug you will use, you can also manipulate the pH to increase efficacy of a drug.
Good luck.
Philippe
Hey Hi,
Literature has proven multiple things that it varies a lot , this and that. But if you think closely:
TUMORS ARE ACIDIC (remember hypoxia there)!!
and if u need a number, its sufficiently low, upto 2-4
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