Dear Colleagues, I would need to know your experience when several concentration of ANG II are applied on HUVECs in vitro, in terms of number of viable cells after incubation. We see about a 30% increase in cell number after 24 hours incubation in different conditions. Could you please send references or share your observations?
Thanks in advance and my best regards
Laura Calvillo
Hello Laura Calvillo
Please refer to the research article below.
The result from the paper below strongly suggest that Ang II is critical for inhibiting HUVEC migration rather than stimulating HUVEC proliferation.
https://www.ahajournals.org/doi/10.1161/01.ATV.0000078521.51319.65?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
HUVEC Proliferation Assay
To avoid bias attributable to angiotensin II–induced HUVEC proliferation, this latter was measured after HUVECs were incubated for 18 hours with increasing angiotensin II concentrations (10−9, 10−7, or 10−6 mol/L). For this purpose, HUVECs were cultured on 96-well flat-bottom microtiter plates and proliferation was assessed by using the MTS colorimetric assay (Cell Titer 96 Aqueous Non-Radioactive Cell Proliferation Assay from Promega), according to manufacturer’s instructions.
Effects of Angiotensin II on HUVEC Proliferation
Using a colorimetric assay for cell proliferation, angiotensin II at 3 different concentrations (10−9, 10−7, and −6 mol/L) had no effects on HUVEC proliferation (after 18 hours of incubation, +1.1±1.9%, +1.5±0.7%, and +2.6±2.3%, respectively; NS versus control), thus excluding a spurious effect of stimulated proliferation on the evaluation of HUVEC migration.
Did you come across this reference ?
It may be helpful.
Best.
Dear Dr. Nobre, thank you very much for your help! I really appreciate it.
My best regards!
- Badges
- Science method