13 September 2023 1 10K Report

Hi, I am culturing HUVECs using the Endothelial Growth Media from PromoCell, however is pretty expensive. Does anyone know any alternative? is it possible to use DMEM for culturing HUVECs?

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Can you suggest me an housekeeping gene for microglia?

I am working with microglia SIMA9 and I will perform a qPCR. If you have already worked with them, can you suggest me one?

05 June 2024 4,930 3 View

Is there any automated catwalk Hydraulic Tray - （catwalks）ajustable resource?

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10 May 2024 8,189 0 View

P21 molecular weight?

I'm working on senescence, and I'm trying to observe senescence markers. I have performed western blots for p21 but it seems that the bands are at a much higher molecular weight than expected. The...

22 March 2024 9,280 2 View

Why iPSC plated for subcloning die after 3 days?

Hi, I am trying to subclone iPSCs by plating 200-300 cells in 6 wells previously coated with geltrex. When I plate them I use 10uM of rock inhibitor and in theory I j keep it until a nice colony...

11 March 2024 1,063 1 View

Is it possible to prepare root samples for SEM when you don't have the critical point dryer equipment?

I have to analyze Eruca sativa roots and leaves. I've seen some methodologies that involve the utilization of the SPI-Dry Critical Point Dryer, however, I don't have access to it. Can I use a Dry...

27 October 2023 5,617 5 View

I'm doing research with the plant model Arabidopsis thaliana, and I've been trying to find information about its NPK content. Does anyone know?

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09 March 2023 9,822 1 View

Which are the best cytokines to use as markers for the senescence associated secretome phenotype (SASP)?

Hello, I'm working on senescence and I'm inducing it using TNF alpha. I would like to assess the induction of the phenotype looking for the cytokines typical of the secretome. Which cytokines do...

19 September 2022 2,258 3 View

Silver fir needles collection and storage?

Hi, I am going for a field campaign to collect needles of Silver fir species, I would like to have a good DNA quality for NGS and I am looking for the best method to preserve the needles in the...

16 August 2022 6,805 0 View

Problems with chromosome spread in summer?

Good evening, I am working on determining the karyotype of a beetle species and performing an in situ hybridization. I have difficulty obtaining good chromosome spread (especially in the summer...

05 July 2022 1,454 0 View

Abnormal high background in chemiluminescense assay?

Hello, we are trying to optimize a microplate assay using chemiluminescence for detection of ABO IgG2. We are experiencing abnormally high background from our reagent blanks (background >20%...

09 December 2021 6,312 1 View

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What could be the possible contaminating organism?

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Media Preparation protocol for Bifidobacteria Agar and Broth?

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BM-MSCs protocols?

Hi Everyone! Currently, I am culturing the BM-MSCs, but it is taking very long, and the yield is very low. I am using DMEM+10% FBS+L-glutamine and I tried the Mesencult media as well. Please let...

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For co-culture HUVEC and muscle cell and use angiogenic and arteriogenic growth factor, what are the markers represent stable blood vessel formation?

We have seen in the literature that Ang-1 can be considered as one of the markers. We need information about few more markers.

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What are these substances forming in my cell culture?

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