Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Can a mRNA be degraded but still observe a protein over-expression?

Hello there, I'm currently trying to over-express a gene by transfecting the cells with a pcDNA6 plasmid containing my target gene. When I measure the mRNA for my gene of interest, the levels are...

03 February 2021 4,112 4 View

Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Where can I purchase NanoLC emitter fused silica picoTip?

Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...

10 January 2021 1,176 4 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Thawing or defrosting methods for frozen soil samples?

I have soil samples that have been frozen for a while and I need to re-acclimate them for a series of analyses that require incubation at 25C. I have been looking for laboratory methods but seem...

04 January 2021 4,862 3 View

Can data collected through participant observation be interpreted through Interpretative phenomenology?

I have just started to read about phenomenology and hence quite a beginner. I have observed hospital interactions between doctors and patients, noted down the conversations, added the non-verbal...

14 January 2021 3,452 3 View

Will there be two research designs if using Mixed Methods?

I am having a hard time with my dissertation pre-proposal (super long story, but I have no help). If I use the Mixed Method, will my research design will be both phenomenological and descriptive...

07 January 2021 5,007 3 View

What are some of the best phenomenological data collection methods for triangulation?

I am hearing impaired, and I am working on my pre-proposal for my dissertation. I am shying away from interviews because of my disability, but I realized there is no way around it. So far, I will...

01 January 2021 5,714 3 View

Qualitative method: phenomenology-based ethnography? Avoiding and prejudgement?

I am struggling to define the methodology for the qualitative approach of my research: I just become familiar with phenomenology-based ethnography but not sure whether is the right choice: My...

10 December 2020 7,892 7 View

Any advice or articles on combining one qualitative approach with the methods of a different approach?

I am a doctoral student and I am looking to do a qualitative research study that looks at the potential influence of teachers on the way minority students view their leadership capabilities....

28 November 2020 6,217 5 View

No title

If it should be a phenomenology study, can the study comprise participants through open-ended survey questions rather than a face-to-face approach? I knew that questions on opinions often belong...

17 November 2020 9,984 10 View

Grounded or phenomenology to study attitudes and perceptions?

hello I am doing my masters in nursing I have qualitative study on perceptions and attitude of moraine use in cancer patients? the author did not clarify the design used? I must identify the...

13 October 2020 9,267 5 View

C. S. Peirce held that, because mathematics makes no presuppositions, it is even more basic than phenomenology? Do you agree?

30 August 2020 3,686 13 View

Theoretical framework suggestions?

Hi, I am in the final stage of my (project) management research, but having trouble finding a theoretical framework that would satisfy my advisor. My research is based on phenomenology with data...

12 August 2020 9,876 2 View

How can we use 'assemblage theory' by multi-disciplines for designing a better children environment?

A research group is conducting a study across several cultures to investigate insights about issues in the environment of school-age children (6-12). The purpose is to explore practitioners'...

05 August 2020 1,967 5 View