Can I calculate catalase activity of HUVEC culture from absorbance?

14 September 2023 0 1K Report

How can I perform the catalase activity assay in adherent cell culture? I plate the cells in a 96-well plate and measure the absorbance at 240nm at 0 and 10 minutes after adding H2O2? Do I need to trypsinize the cells for the reading, or is it okay if they remain adhered to the bottom? And can I read in DMEM medium, or do I need to replace it with PBS when adding H2O2 and taking the reading?

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