How to quantify mitophagy by mtDNA immunostaining (kits or regents)?

02 March 2017 4,779 2 View

What is the golden standard for detection of mitophagy?

What is the golden standard for detection of mitophagy? Thank you

31 December 2016 6,285 3 View

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence?

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence? As reported in literatures, cytochrome C, TOM20 and HSP60 (include many other peoteins of mitochondrion)were...

11 December 2016 9,669 6 View

What is the relationship between sumoylation and deacetylation?

What is the relationship between sumoylation and deacetylation? Do they compete in inhibition or promote each other?

01 February 2015 3,510 4 View

How to treat the relationship between a known target and new target of one drug?

I am very confused how to treat the relationship between a known target and new target of one drug? In other words, recently, our study group found a new target of one drug(we found the drug can...

03 April 2013 4,283 3 View

Can you help with this problem regarding Autoimmune Disease?

Recently, our group did some work in which we looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different approach. So we expect that the 2...

03 April 2013 3,233 9 View

What are the optical properties of the DDX3X protein?

02 March 2013 4,514 2 View

Some problems with autoantibody

Recently, our group did some work which looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different ways. So we expect that the 2 proteins...

02 March 2013 8,156 7 View

Does LPS activate the RIG-1 pathway?

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01 February 2013 7,043 2 View

How to identify the binding site of drug and its target protein?

How to identify the binding site of drug and its target protein?

09 October 2012 1,841 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View