How to quantify mitophagy by mtDNA immunostaining (kits or regents)?

02 March 2017 4,779 2 View

What is the golden standard for detection of mitophagy?

What is the golden standard for detection of mitophagy? Thank you

31 December 2016 6,285 3 View

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence?

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence? As reported in literatures, cytochrome C, TOM20 and HSP60 (include many other peoteins of mitochondrion)were...

11 December 2016 9,669 6 View

What is the relationship between sumoylation and deacetylation?

What is the relationship between sumoylation and deacetylation? Do they compete in inhibition or promote each other?

01 February 2015 3,510 4 View

No title

Thanks in advance for your replies.

11 December 2014 4,441 3 View

How to treat the relationship between a known target and new target of one drug?

I am very confused how to treat the relationship between a known target and new target of one drug? In other words, recently, our study group found a new target of one drug(we found the drug can...

03 April 2013 4,283 3 View

Can you help with this problem regarding Autoimmune Disease?

Recently, our group did some work in which we looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different approach. So we expect that the 2...

03 April 2013 3,233 9 View

What are the optical properties of the DDX3X protein?

02 March 2013 4,514 2 View

Some problems with autoantibody

Recently, our group did some work which looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different ways. So we expect that the 2 proteins...

02 March 2013 8,156 7 View

Does LPS activate the RIG-1 pathway?

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01 February 2013 7,043 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View