How to quantify mitophagy by mtDNA immunostaining (kits or regents)?

02 March 2017 4,779 2 View

What is the golden standard for detection of mitophagy?

What is the golden standard for detection of mitophagy? Thank you

31 December 2016 6,285 3 View

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence?

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence? As reported in literatures, cytochrome C, TOM20 and HSP60 (include many other peoteins of mitochondrion)were...

11 December 2016 9,669 6 View

What is the relationship between sumoylation and deacetylation?

What is the relationship between sumoylation and deacetylation? Do they compete in inhibition or promote each other?

01 February 2015 3,510 4 View

No title

Thanks in advance for your replies.

11 December 2014 4,441 3 View

How to treat the relationship between a known target and new target of one drug?

I am very confused how to treat the relationship between a known target and new target of one drug? In other words, recently, our study group found a new target of one drug(we found the drug can...

03 April 2013 4,283 3 View

Can you help with this problem regarding Autoimmune Disease?

Recently, our group did some work in which we looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different approach. So we expect that the 2...

03 April 2013 3,233 9 View

What are the optical properties of the DDX3X protein?

02 March 2013 4,514 2 View

Some problems with autoantibody

Recently, our group did some work which looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different ways. So we expect that the 2 proteins...

02 March 2013 8,156 7 View

How to identify the binding site of drug and its target protein?

How to identify the binding site of drug and its target protein?

09 October 2012 1,841 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View