Palaniswamy Ramaswamy

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Questions related from Palaniswamy Ramaswamy

For microRNA profiling which method is better RT-qPCR or NGS?

We are planning to do a miRNA profiling to distinguish between two disease conditions with similar and closely associated clinical conditions. Which method of profiling is suitable such as qPCR...

09 September 2017 4,806 8 View

Do you have any suggestions for complete trypsinisation of primary glioma culture?

I have worked with U87MG and U251MG glioma cell lines and trypsinized both the cell lines for 30-40 seconds in 0.05% of trypsin/EDTA soln. Recently I have started doing primary culture from glioma...

12 December 2014 6,108 4 View

Is it ok to use a pooled sample as reference sample in real-time PCR?

I am working on U87 & U251 cell lines. After treatment in triplicates isolated total RNA to cDNA. While doing real-time PCR can I use the pooled cDNA from triplicates as reference sample for...

12 December 2014 1,217 7 View

Which gene should I use as reference gene in Real-time PCR?

Untill now I was doing conventional PCR, there I was using ACTB as internal control and my gene of interest. Now I am planned to use real-time PCR for my gene of interest, where I am expecting...

12 December 2014 3,982 3 View