What is the golden standard for detection of mitophagy?

More Li Hongbin's questions See All

How to quantify mitophagy by mtDNA immunostaining (kits or regents)?

I wanna use a method to quantify mitophagy. However, later on I do not found the detail of mtDNA immunostaining (kits or regents). It will be very lucky If you can provide some details about mtDNA...

02 March 2017 4,876 2 View

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence?

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence? As reported in literatures, cytochrome C, TOM20 and HSP60 (include many other peoteins of mitochondrion)were...

11 December 2016 9,754 6 View

What is the relationship between sumoylation and deacetylation?

What is the relationship between sumoylation and deacetylation? Do they compete in inhibition or promote each other?

01 February 2015 3,602 4 View

What is the difference between different glioma cell lines (eg. A172、U251、U87 and SHG-44)?

Thanks in advance for your replies.

11 December 2014 4,542 3 View

How to treat the relationship between a known target and new target of one drug?

I am very confused how to treat the relationship between a known target and new target of one drug? In other words, recently, our study group found a new target of one drug(we found the drug can...

03 April 2013 4,435 3 View

Can you help with this problem regarding Autoimmune Disease?

Recently, our group did some work in which we looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different approach. So we expect that the 2...

03 April 2013 3,332 9 View

Some problems with autoantibody

Recently, our group did some work which looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different ways. So we expect that the 2 proteins...

02 March 2013 8,246 7 View

What are the optical properties of the DDX3X protein?

What is the absorbance of DDX3X? We want to analyse a drug‘s target protein. After our upstream research, DDX3 is the candidate molecule so we want to detect the change of absorbance of DDX3...

02 March 2013 4,612 2 View

Does LPS activate the RIG-1 pathway?

01 February 2013 7,141 2 View

How to identify the binding site of drug and its target protein?

09 October 2012 1,942 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Maynak Chakraborty

Use kiema vector.If you can show co-localization signal of mitochondria and Atg5, that is one of the indicator of autophagy

https://www.addgene.org/56018/

Christian Q. Scheckhuber

Please refer to the article in the attachment for more detailed information on how to measure mitophagy.

Cheers, Christian

Article Guidelines for the use and interpretation of assays for moni...

Li Hongbin

Thank you everyone