How to quantify mitophagy by mtDNA immunostaining (kits or regents)?

02 March 2017 4,779 2 View

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence?

Which kinds of molecular marker of mitochondrion could be used in immunofluorescence? As reported in literatures, cytochrome C, TOM20 and HSP60 (include many other peoteins of mitochondrion)were...

11 December 2016 9,669 6 View

What is the relationship between sumoylation and deacetylation?

What is the relationship between sumoylation and deacetylation? Do they compete in inhibition or promote each other?

01 February 2015 3,510 4 View

No title

Thanks in advance for your replies.

11 December 2014 4,441 3 View

How to treat the relationship between a known target and new target of one drug?

I am very confused how to treat the relationship between a known target and new target of one drug? In other words, recently, our study group found a new target of one drug(we found the drug can...

03 April 2013 4,283 3 View

Can you help with this problem regarding Autoimmune Disease?

Recently, our group did some work in which we looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different approach. So we expect that the 2...

03 April 2013 3,233 9 View

What are the optical properties of the DDX3X protein?

02 March 2013 4,514 2 View

Some problems with autoantibody

Recently, our group did some work which looked for markers of AIH (autoimmune hepatitis) and we found 2 proteins which upregulated expression in different ways. So we expect that the 2 proteins...

02 March 2013 8,156 7 View

Does LPS activate the RIG-1 pathway?

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01 February 2013 7,043 2 View

How to identify the binding site of drug and its target protein?

How to identify the binding site of drug and its target protein?

09 October 2012 1,841 5 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View