How much RNA should i prepare from my samples for sequencing?

Hi everyone I'm going to extract RNA for sequencing. I want to send the samples to the BGI company for sequencing. How much RNA should I prepare from my samples?

03 April 2018 4,604 3 View

Is there someone who guides me to extract a high quality RNA?

Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)

10 November 2017 8,859 8 View

Can someone introduce me an paper or website for whole genome sequencing?

Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...

09 October 2017 3,040 6 View

What method can be used to obtain the genome of a bacterium from water and milk that has been contaminated with the bacteria?

I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...

04 May 2017 9,363 8 View

How Be used from Circular Dichorism in the drug recombinant proteins?

Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?

31 December 2016 678 8 View

Is the Isoelectric Point of the capsid the same of the protein or does it change?

hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...

31 December 2016 5,080 1 View

In your opinion, What is the best Human housekeeper genes for use in Liver tissue?

hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?

31 December 2016 394 7 View

Is there general signal peptide for plants?

hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...

31 December 2016 1,711 5 View

What is method of finding the cpg islands in promoter region of a gene?

31 December 2016 136 4 View

Can anyone recommend a method to determine if bacteria are hup+ or hup-?

hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.

31 December 2016 4,146 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View