16 Questions 41 Answers 0 Followers
Questions related from Fahimeh Mohammadi
Hi everyone I'm going to extract RNA for sequencing. I want to send the samples to the BGI company for sequencing. How much RNA should I prepare from my samples?
04 April 2018 4,736 3 View
Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)
11 November 2017 8,913 8 View
Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...
10 October 2017 3,103 6 View
I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...
05 May 2017 9,421 8 View
hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.
01 January 2017 4,218 2 View
hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?
01 January 2017 465 7 View
Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?
01 January 2017 741 8 View
hi I need to identify of cpg islands in the number of genes. Is there someone who guide me ؟
01 January 2017 264 4 View
hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...
01 January 2017 1,765 5 View
hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...
01 January 2017 5,143 1 View
Hi Would a 5% polyacrylamide urea gel work?
11 November 2016 1,929 2 View
hi How can I separate plasmid 310kb from gram positive bacteria bacillus anthracis? If we isolate genome, then plasmid comes together. Even all the plasmid isolation procedure showed presence of...
11 November 2016 5,731 3 View
hi Please introduce me a prevention method or an article related to this
11 November 2016 7,188 3 View
HI I have made two RNA's of sizes 1.8 kb and 1.898kb using in vitro transcription method. can I use a 5% PAGE Urea gel to resolve this? I denature my samples at 80 degrees for 95 sec
11 November 2016 6,224 2 View
hiI am searching for antibiotic resistance genes classification systems. Is it possible to classify them?
11 November 2016 4,263 6 View
Hi We have purchased an Addgene plasmid and cannot find out in articles referencing it whether this is an ss or sc vector.How do I quickly find out whether an AAV plasmid is single-stranded or...
10 October 2016 8,238 2 View