Fahimeh Mohammadi

16 Questions 41 Answers 0 Followers

Questions related from Fahimeh Mohammadi

How much RNA should i prepare from my samples for sequencing?

Hi everyone I'm going to extract RNA for sequencing. I want to send the samples to the BGI company for sequencing. How much RNA should I prepare from my samples?

04 April 2018 4,737 3 View

Is there someone who guides me to extract a high quality RNA?

Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)

11 November 2017 8,915 8 View

Can someone introduce me an paper or website for whole genome sequencing?

Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...

10 October 2017 3,104 6 View

What method can be used to obtain the genome of a bacterium from water and milk that has been contaminated with the bacteria?

I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...

05 May 2017 9,422 8 View

Can anyone recommend a method to determine if bacteria are hup+ or hup-?

hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.

01 January 2017 4,221 2 View

In your opinion, What is the best Human housekeeper genes for use in Liver tissue?

hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?

01 January 2017 467 7 View

How Be used from Circular Dichorism in the drug recombinant proteins?

Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?

01 January 2017 743 8 View

What is method of finding the cpg islands in promoter region of a gene?

hi I need to identify of cpg islands in the number of genes. Is there someone who guide me ؟

01 January 2017 265 4 View

Is there general signal peptide for plants?

hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...

01 January 2017 1,766 5 View

Is the Isoelectric Point of the capsid the same of the protein or does it change?

hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...

01 January 2017 5,144 1 View

What is the best way to visualize longer lengths of RNA on gels (>3kb) ?

Hi Would a 5% polyacrylamide urea gel work?

11 November 2016 1,930 2 View

How can I separate large size plasmid (310kb) from gram positive bacteria bacillus anthracis?

hi How can I separate plasmid 310kb from gram positive bacteria bacillus anthracis? If we isolate genome, then plasmid comes together. Even all the plasmid isolation procedure showed presence of...

11 November 2016 5,732 3 View

What is ways to prevent or eliminate of oxidation and deamidation in the monoclonal antibodies ?

hi Please introduce me a prevention method or an article related to this

11 November 2016 7,189 3 View

Is it possible to separate RNA based on its size?

HI I have made two RNA's of sizes 1.8 kb and 1.898kb using in vitro transcription method. can I use a 5% PAGE Urea gel to resolve this? I denature my samples at 80 degrees for 95 sec

11 November 2016 6,225 2 View

Is it possible to classify antibiotic resistance genes? If it is possible, which way should i choice or which algorithm should i use for ?

hiI am searching for antibiotic resistance genes classification systems. Is it possible to classify them?

11 November 2016 4,264 6 View

What is the quickest way to find out whether the ITRs encode for ss (single-stranded) or sc (self-complementary, 'double-stranded') AAV?

Hi We have purchased an Addgene plasmid and cannot find out in articles referencing it whether this is an ss or sc vector.How do I quickly find out whether an AAV plasmid is single-stranded or...

10 October 2016 8,241 2 View