Is there someone who guides me to extract a high quality RNA?

Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)

10 November 2017 8,859 8 View

Can someone introduce me an paper or website for whole genome sequencing?

Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...

09 October 2017 3,040 6 View

What method can be used to obtain the genome of a bacterium from water and milk that has been contaminated with the bacteria?

I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...

04 May 2017 9,363 8 View

How Be used from Circular Dichorism in the drug recombinant proteins?

Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?

31 December 2016 678 8 View

Is the Isoelectric Point of the capsid the same of the protein or does it change?

hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...

31 December 2016 5,080 1 View

In your opinion, What is the best Human housekeeper genes for use in Liver tissue?

hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?

31 December 2016 394 7 View

Is there general signal peptide for plants?

hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...

31 December 2016 1,711 5 View

What is method of finding the cpg islands in promoter region of a gene?

31 December 2016 136 4 View

Can anyone recommend a method to determine if bacteria are hup+ or hup-?

hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.

31 December 2016 4,146 2 View

How can I separate large size plasmid (310kb) from gram positive bacteria bacillus anthracis?

hi How can I separate plasmid 310kb from gram positive bacteria bacillus anthracis? If we isolate genome, then plasmid comes together. Even all the plasmid isolation procedure showed presence of...

10 November 2016 5,667 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View