Hello everyone
I need complete information about CD description in the drug recombinant proteins
Is there anyone who can help me?
You can use CD spectroscopy to study the secondary structure and folding properties of your recombinant protein. Furthermore, you can examine protein interactions.
For more informations:
http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.202.html
Thank you. I do preliminary studies and I know this role CD
But I do not understand its mechanism and how to use it
Hi Fahimeh,
This is the first link that comes up when you do a google search and I've used it to teach my students
https://www.photophysics.com/resources/tutorials/circular-dichroism-cd-spectroscopy
In general, CD give you information about the secondary structure of a protein (helix, sheet, turn/coil). I would assume an experiment studying the CD profile of your protein as a control vs. the same protein recombinant with a drug would tell a great deal about how the drug changes the structure of the protein. More specifically (from my experience) the technique is more sensitive to changes in helicity due to the signature twin dips in the spectra of a helix; meaning the more helical your protein, the more useful CD would be (again, that is a personal opinion from experience)
I hope this helps
CD is a nice tool for certain questions. However, I've noticed that quite often people measure CD spectra just for the sake of it when they don't provide any useful information within their study.
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