I wish to isolate exosomes from MSC conditioned medium and would like to know what is the appropriate temperature for storing it in case i am not proceeding with exosome isolation immediately after conditioned media collection .
1) Can conditioned media be stored at 4C/-20C for a week prior to exosome isolation. Will it damage the exosomes or degrade the biomolecular cargo present within them.?
2) I am aware of the fact that conditioned media can be stored at -80C , but somehow feel uncomfortable doing the same as i think thawing conditioned media from -80 to 4C might damage exosomes resulting in poor exosome yield .
So i need help in two things
1) what is the best temperature (4C/-20C) for short term storage (1-2 weeks) of conditioned media that would cause minimal exosomal damage ?
2) if storing at -80C , what is the best procedure for thawing conditioned media without damaging exosomes..
Thank you