Jonathan D Finn

6 Questions 101 Answers 0 Followers

Questions related from Jonathan D Finn

What is best small ubiquitous promoter?

I am wondering what small (

08 August 2016 4,033 2 View

Do you have any advice for Cas9 purification?

I am thinking about purifying some Cas9 and was wondering if anyone had any tips/suggestions before I start doing some in-depth research. Some specific questions: Tagged vs untagged? (does the tag...

06 June 2015 5,545 3 View

How can one make a more stable mRNA design?

Hello all, I am wondering if anyone knows of any algorithms or work that has been done on rational design of mRNA for increased stability/expression (in eukaryotic cells). I know that AU rich...

11 November 2014 3,912 4 View

Which tissues/organs have high levels of resident macrophages?

I know that macrophages reside in most tissues/organs, but I am wondering if anyone is aware of tissues or organs that have relatively high levels of macrophages (in healthy tissue, not during...

09 September 2014 8,239 25 View

Can anyone help me with immunoprecipitation from plasma?

We are interested in pulling a protein out of mouse plasma and then analyzing by western blot. We have a good sheep antibody that has been used for IP in the past, but not from plasma. We were...

03 March 2014 6,913 8 View

Do FLOW isotype controls really need to be fluorochrome matched?

I know that when doing FLOW it is important to include isotype controls of the same antibody subclass (e.g. IgG1) in order to make sure that your antibody signal is specific and is not being...

03 March 2014 6,731 25 View