16 Questions 23 Answers 0 Followers
Questions related from Shashank Chandanala
i wish to culture monocyte derived macrophages (MDM). Poly d lysine coated dishes are routinely used for this purpose but I would like to coat my culture dishes with poly d lysine instead of...
22 November 2015 4,983 1 View
11 November 2015 9,951 1 View
i wish to culture monocyte derived macrophages (MDM) from PBMC's. Poly d lysine (PDL) coated dishes are routinely used for this purpose but I would like to coat my dishes with PDL instead of...
11 November 2015 7,315 1 View
In the exosome isolation protocol by Thery et al it is recommended to prepare 30 % sucrose cushion using Tris and D2O. I would like to know if the sucrose cushion can be made in PBS instead of...
10 March 2015 3,165 2 View
I wish to isolate exosomes from MSC conditioned medium and would like to know what is the appropriate temperature for storing it in case i am not proceeding with exosome isolation immediately...
03 March 2015 5,781 11 View
03 March 2015 1,845 3 View
I want to reuse the polycarbonate tubes for isolating exosomes. However i can see some sucrose stuck to the bottom of the ultracentrifuge tube from the previous isolation which is not getting...
03 March 2015 1,424 7 View
My hMSCs are failing to adhere to T-25 tissue culture flasks post-thawing. I sterilize my culture flasks by exposing them to hood UV for one hour. Is it good practice to do so, and also does this...
12 December 2014 1,166 9 View
10 December 2014 6,638 8 View
Cargo packaged into exosomes is tightly linked to the behaviour of the cell releasing them. eg a stressed cell would release exosomes with a different protein and RNA composition as opposed to...
18 October 2014 9,290 4 View
I am planning to perform MTT assay for determining the anti-apoptotic activity of MSC derived exosomes on drug treated neurons. Following are my doubts. 1)to collect exosomes should I allow the...
10 October 2014 6,663 4 View
Our lab is finding it difficult to expand MSC's . When transferred from a T-25 flask to a T-75 we observe a marked reduction in their proliferation. They do adhere to the flask but fail to...
10 October 2014 1,632 9 View
I have to isolate exosomes and perform a few functional assays with them. I cannot afford to isolate exosomes every time for performing each single functional assay due to financial constraints....
10 October 2014 1,462 5 View
10 October 2014 9,583 4 View
09 October 2014 1,817 3 View
I am planning to treat HT-22 cell line (neuronal) with NMDA. How do you determine the cell density and NMDA conc. in order to achieve 40%-50 cell death and what precautions must be taken while...
09 September 2014 8,613 5 View
