i wish to culture monocyte derived macrophages (MDM) from PBMC's. Poly d lysine (PDL) coated dishes are routinely used for this purpose but I would like to coat my dishes with PDL instead of buying the commercially available pre coated ones. PDL comes in different molecular weights therefore i would like to know which
1) molecular weight type
2) Poly d lysine concentration
is ideal for culturing macrophages.
And is it an absolute requirement for the dishes to be coated with PDL. What would happen if i culture them on FCS/FBS coated dishes or on polystyrene coated dishes which are negatively charged as opposed to the positively charged poly d lysine.?
~70-150 kD (Sigma P6407) at 50-100 ug/ml. Wash very well after coating as PDL is pretty toxic to cells. More details here:
http://protocolsonline.com/recipes/stock-solutions/polylysine-coated-tissue-culture-surfaces/
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