i wish to culture monocyte derived macrophages (MDM). Poly d lysine coated dishes are routinely used for this purpose but I would like to coat my culture dishes with poly d lysine instead of buying the commercially available pre coated ones. poly d lysine comes in different molecular weights therefore i would like to know which
1) molecular weight type
2) Poly d lysine concentration
is ideal for culturing macrophages.
And is it an absolute requirement for the dishes to be coated with poly d lysine. What would happen if i culture them on FCS/FBS coated dishes or on polystyrene coated dishes which are negatively charged as opposed to the positively charged poly d lysine.?