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Questions related from Perumal Nandhakumar
I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.
12 December 2017 9,236 0 View
I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...
12 December 2017 8,685 0 View
Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...
12 December 2017 6,160 0 View
Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...
12 December 2017 10,057 0 View
During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...
12 December 2017 4,079 0 View
I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...
11 November 2017 3,178 3 View
I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.
11 November 2017 6,095 4 View
I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...
11 November 2017 7,076 0 View
Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...
07 July 2017 3,376 0 View
The pI of Histidine is about 7.5 which is almost equal to the physiological pH. So the total charge of Histidine will be neutral at physiological pH. Then how the His provides positive charge...
05 May 2017 5,966 2 View
will the chilled media can kill the cells ?
04 April 2017 9,614 0 View
I have transfected a eukaryotic expression vector with desire gene in MDA MB cell line. when i was trying to do westernblot, we were getting band in positive control but not in the cell lysate and...
03 March 2017 5,309 5 View
I am about to publish a part of my work in a good international journal. That part includes a modified protocol for assembling multiple gene. Can i publish this work in a high impact journal ? and...
03 March 2017 2,801 2 View
I have made a construct with a target gene and the sequence result was good. is there any chance of mutation in the vector during further plating or subculture what are the precautions should i...
03 March 2017 9,286 3 View
Akt pathway is one among the signal pathway in cancer development and progression. There are many proteins involved in this pathway. in this context i want know whether all the proteins (related...
03 March 2017 3,965 0 View
i am using buffalo endometrail explant tissue for LPA effect.
12 December 2016 6,016 1 View
