05 May 2017 2 6K Report

The pI of Histidine is about 7.5 which is almost equal to the physiological pH. So the total charge of Histidine will be neutral at physiological pH. Then how the His provides positive charge inside the cell ?

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What is the minimum number of amino acids in a peptide to get stain with coomassie brilliant blue?

Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...

11 December 2017 10,011 0 View

Is any article published related to PCR amplification of single (copy) template DNA?

I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.

11 December 2017 9,186 0 View

What is the frequently used SDS PAGE for protein separation of Eukaryotic cell?

I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...

11 December 2017 8,633 0 View

What is the reason for failure of phytocompound in cancer treatment?

Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...

11 December 2017 6,112 0 View

What is the total number of base pair to give signal in real time with sybr green dye?

During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...

11 December 2017 4,027 0 View

What is the minimum ct value for 130 to 200 bp length product in realtime using sybr green?

I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...

10 November 2017 7,023 0 View

What are the common methods frequently used for Characterization and Authentication of Cancer Cell Characterization of Cancer Cell line?

I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.

10 November 2017 6,049 4 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

10 November 2017 3,132 3 View

Is it possible to detect cell line expressed protein using DAB substrate in westernblot ?

Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...

06 July 2017 3,325 0 View

What will happen if i use chilled media to the animal cell line?

will the chilled media can kill the cells ?

03 April 2017 9,571 0 View

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