How the Histidine provides positive charge to the protein ?

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What is the minimum number of amino acids in a peptide to get stain with coomassie brilliant blue?

Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...

11 December 2017 10,114 0 View

What is the frequently used SDS PAGE for protein separation of Eukaryotic cell?

I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...

11 December 2017 8,741 0 View

Is any article published related to PCR amplification of single (copy) template DNA?

I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.

11 December 2017 9,284 0 View

What is the reason for failure of phytocompound in cancer treatment?

Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...

11 December 2017 6,211 0 View

What is the total number of base pair to give signal in real time with sybr green dye?

During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...

11 December 2017 4,141 0 View

What are the common methods frequently used for Characterization and Authentication of Cancer Cell Characterization of Cancer Cell line?

I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.

10 November 2017 6,149 4 View

What is the minimum ct value for 130 to 200 bp length product in realtime using sybr green?

I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...

10 November 2017 7,129 0 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

10 November 2017 3,240 3 View

Is it possible to detect cell line expressed protein using DAB substrate in westernblot ?

Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...

06 July 2017 3,435 0 View

What will happen if i use chilled media to the animal cell line?

will the chilled media can kill the cells ?

03 April 2017 9,664 0 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View