Is there an easy method for liposome preparation?

I'm trying to use new organic compounds as a remedy for sperm membrane injury, I want to use lysosomes as delivering vehicles? can any one give me an easy method for liposome preparation?

03 April 2018 1,462 3 View

Why the absorbency in catalase assay (Aebi method) is unstable?

I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.

10 November 2015 2,533 34 View

Is there an rapid and easy staining procedure for determining sperm morphology?

09 October 2015 2,844 5 View

How I can induce male infertility in mice?

08 September 2015 7,898 14 View

Any one have a free program for IC50 determination in DPPH antioxidant assay?

08 September 2015 3,740 2 View

How do I perform derivative spectroscopy in the assay of lipid conjugated dienes?

I need to assay lipid conjugated dienes in food sample using UV derivative spectroscopy? I have no any information about how to do it?

03 April 2015 4,344 2 View

What is the extraction method for determination of MDA in a meat sample?

See above

08 September 2014 5,872 9 View

In Aebi method for CCATALASE assay.. how I can convert K to International Unit of enzyme

I used Aebi method foe CATALASE assay but the equation result is reaction rate (K), how I can convert it to international unit.

08 September 2014 5,758 0 View

How to convert K to international unit in Aebi method

in the estimation of Catalase the method assay K value, what is the equation to convert it ti IU.

06 July 2014 2,427 0 View

Do you know a method for cyclooxygenase inhibition assay?

Assay of COX inhibition ability of plant extracts.

05 June 2014 10,039 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View