I need a free program or a method for determination of IC50 in DPPH method of antioxidant assay.
Dear Ayad,
Dont know if graphpad is a freeware but if the necessary option is available in free version then you following link may be of help:
http://graphpad.com/support/faq/how-to-determine-an-icsub50sub/
Otherwise, it can be easily calculated in MS Excel by following method:
https://www.youtube.com/watch?v=fMghQw2Ry2c
or you can download an Excel template from following link:
https://www.researchgate.net/publication/253651876_Calculation_of_IC50_an_easy_way_to_calculate_IC50_using_MS_EXCEL
Best Wishes,
Prakash
Data Calculation of IC50: an easy way to calculate IC50 using MS EXCEL
thanks a lot Dear Prakash Katakam
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