Sperm Morpholgy as described by Wyrobek AJ et al, Mutat Res 1983. However, one cannot measure sperm motility by this method.
The smears are fixed in methanol and stained with 10 % Giemsa in Sörensen
buffer for 10 min. A total of 1000 sperms per animal are scored under a microscope with 100x10 magnification. Sperm head abnormalities can be determined as having either normal or abnormal morphology according to Wyrobek and Bruce. According to these criteria, a “hookless head” does not have a spherical spot at the tip of the sperm head; a “banana head” has a banana-like form; an “amorphous head” lacks the usual hook and is deformed; and a “folded sperm” is folded on itself.
References:
1. Wyrobek AJ, Gordon LA, Burkhart JG, Francis MW, Kapp RW Jr, Letz G, Malling HG, Topham JC, Whorton MD. An evaluation of the mouse sperm morphology test and other sperm tests in non-human mammals. A report of the United
States Environmental Protection Agency Gene -Tox Programme. Mutat Res 1983;115:1-72. doi: 10.1016/0165-1110(83)90014-3
2. Wyrobek AJ, Bruce WR. Chemical induction of sperm abnormalities in mice. Proc Natl Acad Sci USA 1975;72:4425-9. doi: 10.1073/pnas.72.11.4425
Although tempting, in my opinion, these rapid stains are not the very best. One can perhaps still use DiffQick, but the Testsimplets I can definitely not recommend.
Regards
There are a mother paper about sperm morphology and it is a very important paper and almost cited around references of papers in this trend. Also it learn you how to differentiation between abnormalities
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC388734/pdf/pnas00062-0239.pdf
Ayad, you might have a look into the existing RG-thread ( 2013ff. ) :
https://www.researchgate.net/post/Can_anyone_tell_the_best_protocol_to_study_the_morphology_of_sperm_in_mice?
find there also e.g. Chicago sky blue....and perhaps also Giemsa...
Best wishes and regards, Wolfgang
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