In Aebi method for CCATALASE assay.. how I can convert K to International Unit of enzyme

09 September 2014 0 6K Report

I used Aebi method foe CATALASE assay but the equation result is reaction rate (K), how I can convert it to international unit.

Badges
Science topic

Similar topics
Cell Biology
Methods

More Ayad Palani's questions See All

Is there an easy method for liposome preparation?

I'm trying to use new organic compounds as a remedy for sperm membrane injury, I want to use lysosomes as delivering vehicles? can any one give me an easy method for liposome preparation?

03 April 2018 1,567 3 View

What is the best method to dissolve DPPH in the aqueous systems ?

I'm working on the use of DPPH in the biological system, I need o dissolve DPPH in buffer system , but DPPH is soluble in methanol and not soluble in aqueous solutions . how can I dissolve it.

01 February 2018 481 0 View

Why the absorbency in catalase assay (Aebi method) is unstable?

I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.

10 November 2015 2,902 34 View

Is there an rapid and easy staining procedure for determining sperm morphology?

I want an rapid and easy staining method for determining sperm morphology.

09 October 2015 2,961 5 View

How I can induce male infertility in mice?

I want to do my experiment o male infertility via inducing it in mice, what is the chemical method for inducing infertility.

08 September 2015 8,009 14 View

Any one have a free program for IC50 determination in DPPH antioxidant assay?

I need a free program or a method for determination of IC50 in DPPH method of antioxidant assay.

08 September 2015 3,897 2 View

How do I perform derivative spectroscopy in the assay of lipid conjugated dienes?

I need to assay lipid conjugated dienes in food sample using UV derivative spectroscopy? I have no any information about how to do it?

03 April 2015 4,445 2 View

What is the extraction method for determination of MDA in a meat sample?

See above

08 September 2014 5,995 9 View

How to convert K to international unit in Aebi method

in the estimation of Catalase the method assay K value, what is the equation to convert it ti IU.

06 July 2014 2,569 0 View

Do you know a method for cyclooxygenase inhibition assay?

Assay of COX inhibition ability of plant extracts.

05 June 2014 10,200 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View