Is there an easy method for liposome preparation?

I'm trying to use new organic compounds as a remedy for sperm membrane injury, I want to use lysosomes as delivering vehicles? can any one give me an easy method for liposome preparation?

03 April 2018 1,462 3 View

What is the best method to dissolve DPPH in the aqueous systems ?

I'm working on the use of DPPH in the biological system, I need o dissolve DPPH in buffer system , but DPPH is soluble in methanol and not soluble in aqueous solutions . how can I dissolve it.

01 February 2018 336 0 View

Why the absorbency in catalase assay (Aebi method) is unstable?

I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.

10 November 2015 2,533 34 View

Is there an rapid and easy staining procedure for determining sperm morphology?

09 October 2015 2,844 5 View

How I can induce male infertility in mice?

08 September 2015 7,898 14 View

Any one have a free program for IC50 determination in DPPH antioxidant assay?

08 September 2015 3,740 2 View

How do I perform derivative spectroscopy in the assay of lipid conjugated dienes?

I need to assay lipid conjugated dienes in food sample using UV derivative spectroscopy? I have no any information about how to do it?

03 April 2015 4,344 2 View

What is the extraction method for determination of MDA in a meat sample?

See above

08 September 2014 5,872 9 View

How to convert K to international unit in Aebi method

in the estimation of Catalase the method assay K value, what is the equation to convert it ti IU.

06 July 2014 2,427 0 View

Do you know a method for cyclooxygenase inhibition assay?

Assay of COX inhibition ability of plant extracts.

05 June 2014 10,039 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View