I want to do my experiment o male infertility via inducing it in mice, what is the chemical method for inducing infertility.
Hi Ayad,
You may want to try N-butyldeoxynojirimycin - male mice become sterile after 3 weeks administration (orally) and recover their fertility within a week off drug. More information here:
Reversible infertility in male mice after oral administration of alkylated imino sugars: A nonhormonal approach to male contraception http://www.pnas.org/content/99/26/17173
Also cadmium (http://www.ncbi.nlm.nih.gov/pubmed/19161232) and ethanol http://www.ncbi.nlm.nih.gov/pubmed/6682442) induce male infertility, but it is not really the way to go in your case.
Hope this helps. Good luck! Norbert
Hi Ayad,
Two points I would like to raise concerning the induction of infertility in male mice by N-butyldeoxynojirimycin. Indeed, this compound will induce infertility in male mice. HOWEVER this only works in a limited number of inbred mouse strains (see my paper http://www.rbej.com/content/5/1/1 and my review, doi: 10.2217/14622416.9.6.717). Essentially, only inbred strains of the C57 family will become infertile (C57BL/6 or /10, C58, C57L, and C57BR). I tried 18 inbred strains in total, and, apart from those strains mentioned here, none became infertile by this drug. Another issue is that N-butyldeoxynojirimycin is rather expensive to buy.
Aarnoud, thanks for providing those details, very interesting! Do you have any idea why the effect of N-butyldeoxynojirimycin is strain-dependent?
I am afraid I do not know the answer to that question. Sore point. I submitted a number of grant applications to address this question, but these proposals were not funded. Even if a genetic screen of some type provides a number of candidate genes, the critical issue is how to evaluate the candidate genes. It is not economical to generate, say, ten KO mouse strains. Experimental support for any allele being involved would be to cross mouse strains that differ in drug sensitivity, and see the drug sensitivity co-segregate with the allele.
Exposure of male mice to estrogens like diethylstilbestrol, ethinylestradiol also results infertility by effecting penis and sperm number and testis also.
Many ways, you can treat with any endocrine disruptor (chemical contraception) which reduces testosterone and increases estradiol level. If you need permanent, then make it surgical (surgical contraception), or can use immunomodulators or antibodies (immunocontraception). Find out your target.
In chemical contraception, there are also many ways using endocrine and reproductive disruptors. Needs to be specified with your objectives and social implications.
Let them join the gem, and they will be sterile as many of our youths.😀
It was a little jock dear. we face many cases of sterility, because of hormonal abuse in sport gym, so this is one of the method to get mice infertility.
regards and happy Ramadhan.