Kathirvel Maruthai

9 Questions 17 Answers 0 Followers

Questions related from Kathirvel Maruthai

What kind of cell line will be most suitable to study TB infection and gene expression in a host?

I am planning to study the epigenetics of TB infection in the host system. I have to quantitatively analyze the expression pattern of certain genes viz., IFN-g, TNF-a, TLR-4, FOXP3...etc. and...

09 September 2014 6,195 13 View

Can someone advise on the genetic analysis of Treg and Tef cells?

I want to study the transcriptional changes in T cells in the context with TB infection. I have a doubt as whether flow cytometry or Flowcytometry with cell sorter will be the best one to do.

10 October 2013 2,355 2 View

How to find the primer binding sequence of a target gene?

I have a handful of primers, of which the details are unknown. In particular, I want to find the exact target sequence bases of the primers in their respective genes. So, I tried to find out, but...

12 December 2012 4,747 7 View

Lack of PCR product visibility in gel?

I ran PCR for the detection of M.tb infection in clinical specimens. Sometimes I get the PCR product as a faint line while running the gel. Could anyone tell me how could I resolve this?

12 December 2012 9,759 8 View

Difficulties in RNA isolation?

I am going to start my protocols in RNA isolation, both qualitative and quantitative. What difficulties should I have in my mind? and what will be the solution for that?

09 September 2012 1,050 7 View

Will proteins get denatured in Ammonium Sulphate precipitation?

While doing extraction of proteins by using ammonium sulphate as salt system, and if it is left to be in salt does it affect the protein nature such as denature, activity lose, changes in...

09 September 2012 2,750 13 View

How to study a complete profile of miRNA/small RNA level in polymorphonuclear (PMC) cells from blood isolate?

I would like to have a complete study about the changes in the expression levels of both miRNA and small RNA from PMC cells isolated from diseased patients blood when compared to normal ones.

08 August 2012 7,348 4 View

Gel Image banding pattern analysis by Dendrogram?

Could anyone tell me how to put dendrogram based on the gel image? I have attached my gel image as sample. What are the possibilities to proceed with?

08 August 2012 1,794 12 View

How to inactivate the action of 5-azacytidine in cell culture to study further DNA methylation effect specifically after infection in same culture?

At first, I am planning to de-methylate (existing methylation) my culture with 5-azaycytidine drug and want to inactivate the further action of this drug. Then I am planning to infect my culture...

01 January 1970 5,733 1 View