What is the aim behind adding "Hi- Di formamide" during the procedure of primer extension or " minisequencing" of DNA"?

More Amjad Hazim Al- Naemi's questions See All

Action to be taken?

Dear RG colleagues, Greetings Today, I received an alert from "Google Scholar" about a new citation to one of my papers. I was highly surprised that those authors have copied long paragraphs from...

01 February 2019 7,566 4 View

Is the "single author" research paper really applicable? How it weighs against the "team- work" research execution& publishing? in medicine mainly?

Apart from "Review Articles", Does being a single author for a scientific paper in a medical specialty stand logic? Still some educational institutions& universities credit "single author...

06 July 2018 2,250 0 View

What's the number "1" criterion to determine the universities ranking by different international organizations& sites?

Every now and then we hear about a new position of a given university or a university college in an international ranking system. What is the most important point in universities ranking? Is it...

06 July 2018 6,467 5 View

Dear RG colleagues, do you support my opinion that reviewing papers as an editorial board member of a given journal has to be a free (non-paid) job?

As publishing scientific papers aims- at the end- to serve humanity and improve the quality of life, I think that the contribution of international reviewers and journals editorials must be free....

05 June 2018 9,222 1 View

Why does "ResearchGate" report a lower number of citations than does other sites like google scholar for the same researcher?

While research sites like google scholar, gives a number of citations for a given researchers' work, RG still provides him/ her a lower citation record.....Also, this sounds the same for the...

05 June 2018 486 4 View

What is the proper action to be honestly taken when a scientific journal accepts a paper& does not publish it for a reason or another?

Some journals may have their activity been ceased for a reason or another for a relatively long time. Prior to this, some papers may have been offered acceptance, but then not published yet!. So,...

05 June 2018 3,184 5 View

To which extent publishing raw data of a started research work or its preprint on researchgate is safe in terms of privacy and copyrights?

Despite that early pre-publishing exposure & sharing of reseach data and/or drafts on researchgate credits the researcher a lot and subjects him for early peer reviewing& citations, but...

03 April 2018 1,639 0 View

Does anybody have an idea that how histone acetyl-transferase& deacetylase play an important role in chromatin remodeling during DNA transcription?

We need to understand the mechanism of chromatin remodeling in order to transcribe DNA. Of these are these two crucial enzymes

02 March 2018 7,956 3 View

I haven't worked on mitochondrial DNA (mtDNA) before. What extra steps would mtDNA extraction needs over genomic DNA extraction? practically i mean?

Extraction of genomic DNA is done easily by some commercial kits. I've done it many times by kits from Promega and Qiagen. What additional steps are needed for extraction of mitochondrial DNA?

02 March 2018 3,439 0 View

What's the lowest number of subjects to be involved in a population study to establish a reference range for a particular serum biochemical analyte?

I am intending to do some work on fasting serum insulin...my aim is to establish its reference range among people of my locality& I would be happy if i can get some useful advises about the...

01 February 2018 9,409 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Adam B Shapiro

To eliminate secondary structure that can interfere with enzyme reactions.

Oxana Karpova

To denaturate qualitatively of the DNA strands for the purpose of following analysis by electrophoresis of single-stranded DNA-fragments of different lengths in POP.

Andrey Shirak

This is highly deionized formamide used to re-suspend samples before electrokinetic injection on capillary electrophoresis systems and prevent sample evaporation.