Action to be taken?

Dear RG colleagues, Greetings Today, I received an alert from "Google Scholar" about a new citation to one of my papers. I was highly surprised that those authors have copied long paragraphs from...

01 February 2019 7,566 4 View

Is the "single author" research paper really applicable? How it weighs against the "team- work" research execution& publishing? in medicine mainly?

Apart from "Review Articles", Does being a single author for a scientific paper in a medical specialty stand logic? Still some educational institutions& universities credit "single author...

06 July 2018 2,250 0 View

What's the number "1" criterion to determine the universities ranking by different international organizations& sites?

Every now and then we hear about a new position of a given university or a university college in an international ranking system. What is the most important point in universities ranking? Is it...

06 July 2018 6,467 5 View

Dear RG colleagues, do you support my opinion that reviewing papers as an editorial board member of a given journal has to be a free (non-paid) job?

As publishing scientific papers aims- at the end- to serve humanity and improve the quality of life, I think that the contribution of international reviewers and journals editorials must be free....

05 June 2018 9,222 1 View

Why does "ResearchGate" report a lower number of citations than does other sites like google scholar for the same researcher?

While research sites like google scholar, gives a number of citations for a given researchers' work, RG still provides him/ her a lower citation record.....Also, this sounds the same for the...

05 June 2018 486 4 View

What is the proper action to be honestly taken when a scientific journal accepts a paper& does not publish it for a reason or another?

Some journals may have their activity been ceased for a reason or another for a relatively long time. Prior to this, some papers may have been offered acceptance, but then not published yet!. So,...

05 June 2018 3,184 5 View

To which extent publishing raw data of a started research work or its preprint on researchgate is safe in terms of privacy and copyrights?

Despite that early pre-publishing exposure & sharing of reseach data and/or drafts on researchgate credits the researcher a lot and subjects him for early peer reviewing& citations, but...

03 April 2018 1,639 0 View

I haven't worked on mitochondrial DNA (mtDNA) before. What extra steps would mtDNA extraction needs over genomic DNA extraction? practically i mean?

Extraction of genomic DNA is done easily by some commercial kits. I've done it many times by kits from Promega and Qiagen. What additional steps are needed for extraction of mitochondrial DNA?

02 March 2018 3,439 0 View

What's the lowest number of subjects to be involved in a population study to establish a reference range for a particular serum biochemical analyte?

I am intending to do some work on fasting serum insulin...my aim is to establish its reference range among people of my locality& I would be happy if i can get some useful advises about the...

01 February 2018 9,409 8 View

What is the aim behind adding "Hi- Di formamide" during the procedure of primer extension or " minisequencing" of DNA"?

Addition of Hi-Di formamide is traditionally necessary during primer extension procedure prior to samples loading in a gene analyzer...what does this step stand for?

01 February 2018 1,610 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View