Extraction of genomic DNA is done easily by some commercial kits. I've done it many times by kits from Promega and Qiagen.
What additional steps are needed for extraction of mitochondrial DNA?
Dear RG colleagues, Greetings Today, I received an alert from "Google Scholar" about a new citation to one of my papers. I was highly surprised that those authors have copied long paragraphs from...
01 February 2019 7,566 4 View
Apart from "Review Articles", Does being a single author for a scientific paper in a medical specialty stand logic? Still some educational institutions& universities credit "single author...
06 July 2018 2,250 0 View
Every now and then we hear about a new position of a given university or a university college in an international ranking system. What is the most important point in universities ranking? Is it...
06 July 2018 6,467 5 View
As publishing scientific papers aims- at the end- to serve humanity and improve the quality of life, I think that the contribution of international reviewers and journals editorials must be free....
05 June 2018 9,222 1 View
While research sites like google scholar, gives a number of citations for a given researchers' work, RG still provides him/ her a lower citation record.....Also, this sounds the same for the...
05 June 2018 486 4 View
Some journals may have their activity been ceased for a reason or another for a relatively long time. Prior to this, some papers may have been offered acceptance, but then not published yet!. So,...
05 June 2018 3,184 5 View
Despite that early pre-publishing exposure & sharing of reseach data and/or drafts on researchgate credits the researcher a lot and subjects him for early peer reviewing& citations, but...
03 April 2018 1,639 0 View
We need to understand the mechanism of chromatin remodeling in order to transcribe DNA. Of these are these two crucial enzymes
02 March 2018 7,956 3 View
I am intending to do some work on fasting serum insulin...my aim is to establish its reference range among people of my locality& I would be happy if i can get some useful advises about the...
01 February 2018 9,409 8 View
Addition of Hi-Di formamide is traditionally necessary during primer extension procedure prior to samples loading in a gene analyzer...what does this step stand for?
01 February 2018 1,610 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View