What is the acceptable Passage number of MDA-MB-231cells?

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Protein Synthesis technique?

What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine? Thank you

31 December 2018 4,075 3 View

What is the most dependable (reliable) factor to determine the tumour microenviroment difference between different types of cancer?

The tumour microenviroment is very complex and composed from many bio-factors such as (EGFR, VEGF, chemokines, leuckocytes infilitrations...etc). I did many investigations on couple of tumour...

11 December 2018 579 0 View

Can the micro-motion in dental implant affect the occlussion, and unnoticed bone dehiscence?

Despite the limited motion, but I think in cases of crowded teeth or lateral interferences, these may result in future occlussion problems.

10 November 2018 7,453 5 View

Proteasome Inhibitor that blocks Caspase-like activity?

Hi, Any suggestion for proteasomal inhibitor that blocks caspase-like activity of the proteasome in mammalian cells? MG132 and Bortezomib both acts on ChT-like acitvity! Thanx

06 November 2018 7,205 1 View

Bromophenol blue for 6X loading protein buffer?

Hello, In order to prepare the Bromophenol blue for 6X loading buffer for protein. Do you use ethanol to dissolve Bromophenol blue or PBS buffer. I made with ethanol but my gel bands started to...

01 February 2018 349 0 View

Time of MDA-MB-231 cell starvation in serum free media?

Hi, How long I need to starve MDA-MB-231 cells in serum free media to synchronize them before I infect them with my protein of interest using Adenovirus.. I'm aiming at conducting cell cycle...

22 December 2017 3,086 3 View

Bioluminescence for tumor metastasis?

Hello, What are the limitations and pitfalls of using luceiferase expressing cancer cells injected in Xenographt mice for studying the metastasis? Thank you

18 November 2017 7,480 2 View

Cell Viability Assay Standardization?

Hi, How do you normalize your cell numbers in viability assay to the zero hour reading if we start with different number of cells, because after seeding cells in 96 hr, there are different cell...

08 September 2017 7,908 3 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

16 July 2017 2,045 1 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

06 July 2017 9,306 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View