Protein Synthesis technique?

What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine? Thank you

31 December 2018 3,911 3 View

What is the most dependable (reliable) factor to determine the tumour microenviroment difference between different types of cancer?

The tumour microenviroment is very complex and composed from many bio-factors such as (EGFR, VEGF, chemokines, leuckocytes infilitrations...etc). I did many investigations on couple of tumour...

11 December 2018 452 0 View

Can the micro-motion in dental implant affect the occlussion, and unnoticed bone dehiscence?

Despite the limited motion, but I think in cases of crowded teeth or lateral interferences, these may result in future occlussion problems.

10 November 2018 7,331 5 View

Bromophenol blue for 6X loading protein buffer?

Hello, In order to prepare the Bromophenol blue for 6X loading buffer for protein. Do you use ethanol to dissolve Bromophenol blue or PBS buffer. I made with ethanol but my gel bands started to...

01 February 2018 217 0 View

Cell Viability Assay Standardization?

Hi, How do you normalize your cell numbers in viability assay to the zero hour reading if we start with different number of cells, because after seeding cells in 96 hr, there are different cell...

08 September 2017 7,784 3 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

16 July 2017 1,907 1 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

06 July 2017 9,149 1 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

04 July 2017 8,112 3 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

12 June 2017 9,422 1 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

05 June 2017 9,866 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View