Protein Synthesis technique?

What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine? Thank you

31 December 2018 3,911 3 View

What is the most dependable (reliable) factor to determine the tumour microenviroment difference between different types of cancer?

The tumour microenviroment is very complex and composed from many bio-factors such as (EGFR, VEGF, chemokines, leuckocytes infilitrations...etc). I did many investigations on couple of tumour...

11 December 2018 452 0 View

Can the micro-motion in dental implant affect the occlussion, and unnoticed bone dehiscence?

Despite the limited motion, but I think in cases of crowded teeth or lateral interferences, these may result in future occlussion problems.

10 November 2018 7,331 5 View

Cell Viability Assay Standardization?

Hi, How do you normalize your cell numbers in viability assay to the zero hour reading if we start with different number of cells, because after seeding cells in 96 hr, there are different cell...

08 September 2017 7,784 3 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

16 July 2017 1,907 1 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

06 July 2017 9,149 1 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

06 July 2017 8,514 4 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

04 July 2017 8,112 3 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

12 June 2017 9,422 1 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

05 June 2017 9,866 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View