Tariq Khan

4 Questions 82 Answers 0 Followers

Questions related from Tariq Khan

Why or how does serum effects the transfection efficiency?

Transfection using siRNA for cell lines.

05 May 2018 6,609 1 View

Anyonw who knows about Standard/Optimized protocol for clonogenic assay using siRNA?

Colony formation capacity of cells suing siRNA. As cells are incubated for 7 to 14 days, by adding FBS it reduces or revert transfection. Please find attached my optimized protocol.

05 May 2018 6,783 0 View

Stripping of blot/ removal of primary antibody?

I have used mild as well as harsh stripping buffer. Also, 5mM EDTA stripping for western blot. Anyone here who can suggest, best buffer for primar antibody removal from blot along with protocol....

04 April 2018 2,856 0 View

Looking for PhD position open in Oncology or Reproductive biology

I have overall 2 years of Research experience including my Master thesis project. I have co authored 2 publication in international journal. One poster in international conference. I have cleared...

01 January 1970 2,164 6 View