Sarmad Al-Marsoummi

6 Questions 4 Answers 0 Followers

Questions related from Sarmad Al-Marsoummi

Protein Synthesis technique?

What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine? Thank you

01 January 2019 4,035 3 View

Bromophenol blue for 6X loading protein buffer?

Hello, In order to prepare the Bromophenol blue for 6X loading buffer for protein. Do you use ethanol to dissolve Bromophenol blue or PBS buffer. I made with ethanol but my gel bands started to...

02 February 2018 326 0 View

Cell Viability Assay Standardization?

Hi, How do you normalize your cell numbers in viability assay to the zero hour reading if we start with different number of cells, because after seeding cells in 96 hr, there are different cell...

09 September 2017 7,868 3 View

What is the acceptable Passage number of MDA-MB-231cells?

Hi, I'm wondering what passage number is acceptable for MDA-MB-231 cells, Some labs kill these cells when they are 20-25 passages. Others Suggest it should be below 60 passages Thanks

07 July 2017 8,616 4 View

STAT3 alpha isofrom siRNA

Hi Anybody was able to use siRNA to block STAT3 alpha specifically? Thanx

07 July 2017 9,262 1 View

Hi I want to synchronize my MDA-Mb-231 cells and infect them with Adenovirus with protein of interest any suggested protocol?

I used MDa-MB-231 cells, interested in protein that I want to overexpress by using Adenovirus I want to synchronize the cells to do cell cycle analysis Any Suggested steps ? Thanx

06 June 2017 9,967 1 View