What method can you use to measure protein synthesis(translation) of specific protein in cell culture as an alternative to radio-labeled Methionine?
Thank you
do you want to measure translation of a single transcript and corresponding peptide, or global translation? For global translation there is AHA (methionine analog) and OPP-puromycin labeling kits that use 'click-chemistry' reaction to attach a label for visualization of protein synthesis.
https://www.thermofisher.com/order/catalog/product/C10456
alternatively, if you want to look at translation of single proteins, there is a SILAC-MS/MS approach, as well as a a fluorescent imaging method. below are the papers describing these methods.
https://www.cell.com/neuron/fulltext/S0896-6273(18)30472-0
http://science.sciencemag.org/content/352/6292/1430/tab-figures-data
Patrick thank you very much.. Yes I'm trying to measure a single protein translation
Immunological techniques, such as Western blot or ELISA. You need an antibody specific for that protein. To make such an assay quantitative, you should have a standard, which is the purified protein.
If it is an enzyme with an activity that is very specific, then you might be able to do an enzyme activity assay.
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