How to exclude direct effect of drugs to neurons in mouse parkinson's model?

26 October 2020 1,127 2 View

LC3I band detection?

Hi, I have some questions about the electrophoresis and transfer condition. In our lab, we used 15% gel to SDS-page and semi-dry transfer In electrophoresis, i migrated the stacking gel in 80 V...

14 October 2020 4,046 2 View

How can i solve this problem, OPV active film getting hazy?

Hi, guys.. These days, I have some problem in OPV processing. To be more specific, the active film is OK right after spin casting. But while drying it, the film is getting hazy..... it is changed...

07 January 2020 7,673 4 View

How to make SnO2 thin film by SnCl2-2H2O?

26 November 2019 5,407 3 View

What size of gold nanoparticles should I synthesize?

13 July 2019 5,878 2 View

how high temperature pepper fruits can resist?

I hope to monitor how fungal conidia (colletotrichum acutatum) infect on dead pepper fruits. so, I dipped sliced fragments of a pepper fruit in boiling water for 30 seconds and inoculated fungal...

11 July 2019 6,618 3 View

Is it appropriate to use DMSO:Corn oil (50:50) in mouse as IP?

Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...

03 December 2018 4,092 1 View

Do you know reason for wrong output on HOMO and LUMO energies in Gamess?

In the output result, I found that HOMO and LUMO energies were too high. I wonder what is the problem in here. Please check attached files. In first file, NPREO was used to print homo-1, homo,...

23 August 2018 7,738 7 View

Has anybody tried the cellulose-based DNA clean-up method?

Hello colleagues, Nucleic acid purification from plants, animals and microbes ... I wonder if this method works in other people's hands, particularly for adherent mammalian cells. I also wonder...

04 May 2018 3,729 0 View

The usage of clock circuit in micro controller chip?

Why do we use clock circuit when we are designing certain micro controller onto PCB? What it is usage? What other circuits do we need in order to proceed? Would you be able to give some advice...

03 January 2018 8,975 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View