What is DNA sequencing ?
DNA has the code
for a person. The code
comes from the father
and the mother. But how
can we read this code?
Here's a great paper in Nature explaining past and present DNA sequencing technologies:
https://www.nature.com/articles/nature24286
Ziad Sobih
try this
https://lmgtfy.com/?q=%22What+is+DNA+sequencing%22
Dear Ziad
The term DNA sequencing refers to methods for determining the order of the nucleotides bases adenine,guanine,cytosine and thymine in a molecule of DNA. The first DNA sequence were obtained by academic researchers,using laboratories methods based on 2- dimensional chromatography in the early 1970s. In 1973 , Gilbert and Maxam reported the sequence of 24 base pairs using a method known as wandering- spot analysis. The chain termination method developed by Sanger and coworkers in 1975 owing to its relative easy and reliability. In 1975 the first complete DNA genome to be sequenced is that of bacteriophage X174. A sequencing can be done by different methods:
1. Maxam – Gilbert sequencing
2. Chain-termination methods
3. Dye-terminator sequencing
4. Automation and sample preperation
5. Large scale sequencing strategies
6. New sequencing methods.
DNA molecules are made from some 'building blocks' [designed 'A', 'T', 'C' and 'G'] (like bricks for a house). DNA sequencing is using a machine to sequence a particular DNA molecules to find out those building blocks' orders. Ex. ATTTCGAG or GTCAGGATT etc.
The order of the building blocks (ATGC), in the sample or target amplicon of u want to know the sequence.
For what purpose u want do DNA sequencing? Depending on your application you can choose your platform or sequencing meyhodolgy
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