is it possible to do mycoplasma contamination test in my Warthon jelly tissues frozen at -80°C ? all what i found n the internet is compatible with cell culture supernatant and nothing about tissues. Thank you by advance
Hello Ziad Hassan
Mycoplasma contamination is predominantly found in cultured samples (e.g. cell lines) and not in non-cultured samples (e.g. tissues).
I have attached a few articles below for your reference.
https://academic.oup.com/nar/article/43/5/2535/2453278
Article Mycoplasma infection significantly alters microarray gene ex...
https://www.future-science.com/doi/full/10.2144/000113271?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org
Best.
Yes, it is possible to test for mycoplasma contamination in Warthon jelly tissues that have been frozen at -80°C. However, it is important to note that mycoplasma testing in tissue samples is more challenging than testing in cell culture supernatant. This is because mycoplasma can reside within the cells and not just in the culture media, making detection more difficult.
There are several methods for testing mycoplasma contamination in tissue samples, including PCR-based methods, culture-based methods, and serological methods. The choice of method depends on the specific needs of the laboratory and the characteristics of the samples.
When using PCR-based methods, it is important to extract DNA from the tissue samples prior to testing. The extracted DNA can then be used as a template for PCR amplification of mycoplasma DNA sequences. PCR-based methods are highly sensitive but may not be able to distinguish between live and dead mycoplasma.
Culture-based methods involve transferring the tissue samples to a culture medium that supports the growth of mycoplasma. This method can be time-consuming and may not detect mycoplasma in samples that have been stored for a long time or have been subjected to repeated freeze-thaw cycles.
Serological methods involve testing for antibodies to mycoplasma in the tissue samples. This method can be useful for detecting mycoplasma infection in tissues, but may not be suitable for detecting low levels of mycoplasma contamination.
It is recommended to consult with a laboratory specialist or a mycoplasma expert for guidance on the best approach for testing mycoplasma contamination in your tissue samples.
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