The future of stem cells in brain repair??

09 May 2024 0 8K Report

The future of stem cells in brain repair and TBI???

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Why do I get different results between Coomassie Gels and Western Blot?

Hi everyone, I hope you're doing well. I've encountered an interesting phenomenon in my research and would appreciate your insights. When running gels and staining them with Coomassie, I'm...

18 March 2024 3,416 0 View

Why am I unable to detect pERK in HEK293T cells through Western Blotting?

Greetings to all, I have attempted to detect pERK using western blotting with HEK 293T cells, but I am unable to do so. I overexpressed a gene that caused pERK to be overproduced, and I was...

21 December 2023 7,195 3 View

Inner geometry suppression when refining the mesh?

Dear ICEM CFD users, Could you please help me to solve this problem? I have a inner geometry inside an other. A coarse mesh of such a configuration captures the inner geometry but a refined mesh...

25 September 2023 2,887 0 View

Do conifers produce tracheid with spiral thickness? If not, Is tracheids spiral thickness the same thing as the "Cellulose Microfibril Angle"?

Hello, Tracheid in Cupressaceae and other conifers may exhibit ornamentation on their walls that resemble helical spiral thickness little bit, as result of an angle in Microfibrils. What is the...

22 January 2023 5,334 2 View

How can i do Mycoplasma test in tissues ?

is it possible to do mycoplasma contamination test in my Warthon jelly tissues frozen at -80°C ? all what i found n the internet is compatible with cell culture supernatant and nothing about...

30 November 2022 6,320 3 View

How do I interpret my MTT assay results, and what statistical tests should I do for cell viability?

I have completed an experiment where I exposed rainbow trout liver cells to copper concentrations of 0.01, 0.1, 1.0 M, along with a negative control. I have recorded the cell viability % at each...

20 March 2021 9,445 7 View

Can any one explain why the ethyl ester Biodiesel (FAEE) has higher than that of the metyl ester FAME- both of them are derived from the same oil?

Biodiesels derived from waste vegetable oil transesterification with Ethanol (FAEE) show higher peroxide values than those derived by transesterification with methanol (FAME) prepared under the...

13 February 2021 515 6 View

I need a help from a spring technician or some who know a lot about heavy duty spring ?

I need a spring technician or some who know a lot about heavy duty spring to ask him/her a couple of questions in my bachelor thesis. The question will be in direction of choosing the right...

20 December 2020 8,646 0 View

What happens to a gas after compressing it for 10 years?

Will it lose a fraction of its expansion power assuming it was insulated in this system? Because I am designing an insulated gas spring. what I want to know if the gas will lose some of its...

07 December 2020 6,363 3 View

What about the protocol used for president Trump for treating COVID-19?

The protocol used in managing COVID-19 for Mr. president Trump containing: Regenerons poly-clonal antibody cocktail Zinc Vit. D Fomatidine Melatonin Aspirin what are the differences and...

09 October 2020 7,799 3 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View