What is an easy protocol to purify a protease from zymography gel?

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Metagenomic analysis of bacteria - where to start?

I have no knowledge about metagenomic analysis. Could you suggest me an article or a website to start with from scratch? Please suggest FROM YOUR PERSONAL EXPERIENCE! Thanks!

06 July 2019 3,555 10 View

Is it OK to use AutoAnalyzer or BioAnalyzer for the measurement of cholesterol in bacterial culture media?

I would like to add cholesterol in bacterial growth media and determine cholesterol concentration in the presence and absence of some isolated bacteria. Is it OK to do it by the Auto-Analyzers /...

06 July 2019 9,570 1 View

What reagent can be used to kill bacteria?

What are the reagents that can be used to kill bacterial cells in a culture or culture-supernatant without affecting the proteins (and other biomolecules) secreted by the bacteria?

04 May 2019 2,178 4 View

Why no trace elements in bacterial minimal media?

Why are trace elements or vitamins not included in some of the bacterial minimal media like Bushnell Hans media? Are trace elements not essential for bacterial growth? If, for example, any carbon...

04 May 2019 7,159 16 View

How to manually setup anerobic and microaerophilic system(s) to cultivate Lactic Acid Bacteria?

If I cannot use the commercially available ones? Thanks!

02 March 2019 9,978 12 View

Are bacteria isolated from the gut of marine fishes called marine bacteria?

Can bacteria isolated from the gut of a marine fish be termed as marine bacteria? In other words, are these bacteria regarded as bacteria of marine environment?Are such bacteria similar to those...

01 February 2019 8,939 16 View

Do bacteria grow under water?

Do bacteria grow in soil that remains under water all the time? Or, are bacteria growing under water all anaerobes? If not, where from do they get O2 if there is not much agitation for O2 to be...

31 December 2018 3,538 7 View

How is the identification of Lactic Acid Bacteria (LAB) confirmed?

Lactic Acid Bacteria (LAB) have some unique characteristics, e.g, being gram-positive, catalase-negative, anaerobic, halo surrounding. But how to confirm that any bacteria isolated from any...

05 June 2018 2,563 4 View

To determine acid tolerance, bacterial growth is monitored by calculating CFU on agar plates, why not by measuring absorbency/OD?

While checking acid tolerance of bacteria, the growth of the culture in acid media is monitored by plating the culture on agar plates and then counting the CFUs. Why isn't it done...

04 May 2018 2,114 6 View

Is it OK to use NanoDrop A280 for total protein estimation in culture supernatant?

I would like to measure protease/amylase/lipase/cellulase activity in culture supernatant of different bacteria. To compare any of these enzyme activity in the supernatant of the different...

02 March 2018 2,870 7 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View