Metagenomic analysis of bacteria - where to start?

I have no knowledge about metagenomic analysis. Could you suggest me an article or a website to start with from scratch? Please suggest FROM YOUR PERSONAL EXPERIENCE! Thanks!

06 July 2019 3,555 10 View

Is it OK to use AutoAnalyzer or BioAnalyzer for the measurement of cholesterol in bacterial culture media?

I would like to add cholesterol in bacterial growth media and determine cholesterol concentration in the presence and absence of some isolated bacteria. Is it OK to do it by the Auto-Analyzers /...

06 July 2019 9,570 1 View

Why no trace elements in bacterial minimal media?

Why are trace elements or vitamins not included in some of the bacterial minimal media like Bushnell Hans media? Are trace elements not essential for bacterial growth? If, for example, any carbon...

04 May 2019 7,159 16 View

How to manually setup anerobic and microaerophilic system(s) to cultivate Lactic Acid Bacteria?

If I cannot use the commercially available ones? Thanks!

02 March 2019 9,978 12 View

Are bacteria isolated from the gut of marine fishes called marine bacteria?

Can bacteria isolated from the gut of a marine fish be termed as marine bacteria? In other words, are these bacteria regarded as bacteria of marine environment?Are such bacteria similar to those...

01 February 2019 8,939 16 View

Do bacteria grow under water?

Do bacteria grow in soil that remains under water all the time? Or, are bacteria growing under water all anaerobes? If not, where from do they get O2 if there is not much agitation for O2 to be...

31 December 2018 3,538 7 View

How is the identification of Lactic Acid Bacteria (LAB) confirmed?

Lactic Acid Bacteria (LAB) have some unique characteristics, e.g, being gram-positive, catalase-negative, anaerobic, halo surrounding. But how to confirm that any bacteria isolated from any...

05 June 2018 2,563 4 View

To determine acid tolerance, bacterial growth is monitored by calculating CFU on agar plates, why not by measuring absorbency/OD?

While checking acid tolerance of bacteria, the growth of the culture in acid media is monitored by plating the culture on agar plates and then counting the CFUs. Why isn't it done...

04 May 2018 2,114 6 View

Is it OK to use NanoDrop A280 for total protein estimation in culture supernatant?

I would like to measure protease/amylase/lipase/cellulase activity in culture supernatant of different bacteria. To compare any of these enzyme activity in the supernatant of the different...

02 March 2018 2,870 7 View

What is an easy protocol to purify a protease from zymography gel?

I need a protocol to purify protease (present in culture supernatant) from casein or gelatin zymogram Gels. How efficient would it be considering the amount of the protease to be purified? Can the...

10 November 2017 8,946 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View