I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and should not melt any more, but I read protocols with solving gelatin out of sections in warm PBS.
Can someone give me an answer based on practical experience?
Hello Gudrun Lang,
My advisor tried to embed in paraffin some embryos few years ago through a method that uses gelatin published in: Article Paraffin-embedded manipulated blastocysts: A tool to demonst...
In the HE-stained sections, it was possible to see the gelatin around the embryo, meaning that it did not melt in the water bath at 45–50 °C after sectioning. Even in the Zech et al. paper, you will see the gelatin in the images.
I hope this helps
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