If I cannot use the commercially available ones? Thanks!
Thanks Dr. Alaa Kareem Niamah and Dr. Ali Al‐Hisnawi .Is plastic jar ok to use?
したくないです 私の名前を入力し with the plastic jar used gas pak but with glass jar used candle because fir effect on plastic
Dear Dr. Alaa Kareem Niamah, I have used a glass jar with a candle but it was overgrown by fungus after 24 hours incubation. It happened for many times. Is there any other alternative way? thank you.
You can use thioglycolate media with added oil (glycerol for example), you should choose a good type of plastic or glass jar for incubation.
Also, you can use any antifungal agent with MRS media to prevent growth of candida
1: For a Lab-made anaerobic jar, first of all you need to confirm the anaerobiosis. I used Methylene blue (1%) with a candle for confirmation of anaerobic environment inside the jar; where methylene blue turns colourless if your Jar is completely anaerobic and remains light blue if there are any O2 traces left. I would suggest to use a glass jar (5 mm thickness, 1 L) with an airtight head.
2: You can also use the FTM (fluid thioglycollate medium) to check the oxygen demand of the strains. I used screw tights glass Vials for the said purpose and got very clear results. The strict anaerobes grows a the bottom of the FTM culture Vials only.
Thanks a lot Syed Salman Ali Shah for your help!
Could you let me know:
1. How did you use the methylene blue solution?
2. For the FTM, if I keep some portion of the test tube empty as we usually do, is it required to remove air from the empty space above the media in the tube?
1: I have covered this in my earlier answer.
2: You can purge it with N2 gas to ensure anaerobiosis for long period if your culture needs more time than usual i-e more than 96 hours. As FTM has redox indicator in the recipe, so you can clearly differentiate between aerobic and anaerobic regions inside the culture tube (pink colour region = oxygen presence).
I hope this helps you find the way to achieve your desired goals.
Please let me know if you need any further information.
Best,
Shah.
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