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Questions related from したくないです 私の名前を入力し
I would like to add cholesterol in bacterial growth media and determine cholesterol concentration in the presence and absence of some isolated bacteria. Is it OK to do it by the Auto-Analyzers /...
07 July 2019 9,662 1 View
I have no knowledge about metagenomic analysis. Could you suggest me an article or a website to start with from scratch? Please suggest FROM YOUR PERSONAL EXPERIENCE! Thanks!
07 July 2019 3,673 10 View
Why are trace elements or vitamins not included in some of the bacterial minimal media like Bushnell Hans media? Are trace elements not essential for bacterial growth? If, for example, any carbon...
05 May 2019 7,248 16 View
What are the reagents that can be used to kill bacterial cells in a culture or culture-supernatant without affecting the proteins (and other biomolecules) secreted by the bacteria?
05 May 2019 2,280 4 View
If I cannot use the commercially available ones? Thanks!
03 March 2019 10,058 12 View
Can bacteria isolated from the gut of a marine fish be termed as marine bacteria? In other words, are these bacteria regarded as bacteria of marine environment?Are such bacteria similar to those...
02 February 2019 9,027 16 View
Do bacteria grow in soil that remains under water all the time? Or, are bacteria growing under water all anaerobes? If not, where from do they get O2 if there is not much agitation for O2 to be...
01 January 2019 3,630 7 View
Lactic Acid Bacteria (LAB) have some unique characteristics, e.g, being gram-positive, catalase-negative, anaerobic, halo surrounding. But how to confirm that any bacteria isolated from any...
06 June 2018 2,638 4 View
While checking acid tolerance of bacteria, the growth of the culture in acid media is monitored by plating the culture on agar plates and then counting the CFUs. Why isn't it done...
05 May 2018 2,205 6 View
I would like to measure protease/amylase/lipase/cellulase activity in culture supernatant of different bacteria. To compare any of these enzyme activity in the supernatant of the different...
03 March 2018 2,968 7 View
I need a protocol to purify protease (present in culture supernatant) from casein or gelatin zymogram Gels. How efficient would it be considering the amount of the protease to be purified? Can the...
11 November 2017 9,024 2 View
