Metabolism of mice ?

Compared to humans, what are the metabolic (biochemical) features and differences ? What is important when we study the metabolic of mice and want to extrapolate to humans ?

09 October 2014 2,514 0 View

How can you decide drug quantities given to mice based on the weight of the animal?

09 October 2014 9,125 5 View

What is the best animal choice?

For a pharmacological and another pathway study related to Alzheimer's disease (such as those including building animal models), what is the best animal choice? The mice or the rats? If it is the...

08 September 2014 7,505 6 View

What would be the best animal model to study drug effects on Alzheimer's Disease?

08 September 2014 2,114 4 View

Can anyone provide advice on the study of APOE transgenic cell lines ?

To study Alzheimer's disease , I need to study the involvement of APOE genes in Amyloid Beta production thus, I am searching for suitable cell lines that specifically express these genes (APOE 2,...

08 September 2014 2,438 2 View

Can we extrapolate pharmacological data from mice to rats and vice versa?

08 September 2014 2,005 10 View

Do you have protocols of primary cells cultures of hippocampal and cortical cells respectively (taken from monkey brain)?

See above

07 August 2014 845 1 View

What are the best choices for the cell culture to study Alzheimer's disease?

07 August 2014 1,880 8 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View