What are the best choices for the Cell culture to study Alzheimer;s disease ?

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Metabolism of mice ?

Compared to humans, what are the metabolic (biochemical) features and differences ? What is important when we study the metabolic of mice and want to extrapolate to humans ?

09 October 2014 2,598 0 View

How can you decide drug quantities given to mice based on the weight of the animal?

Since the drug quantity given to mice depends on the animal weight: 1. How often we have to weigh the animal to adjust? Once a week? 2. Is it acceptable if in the same group that has around 8 mice...

09 October 2014 9,218 5 View

What is the best animal choice?

For a pharmacological and another pathway study related to Alzheimer's disease (such as those including building animal models), what is the best animal choice? The mice or the rats? If it is the...

08 September 2014 7,627 6 View

Can anyone provide advice on the study of APOE transgenic cell lines ?

To study Alzheimer's disease , I need to study the involvement of APOE genes in Amyloid Beta production thus, I am searching for suitable cell lines that specifically express these genes (APOE 2,...

08 September 2014 2,643 2 View

Can we extrapolate pharmacological data from mice to rats and vice versa?

When we have a therapeutic dose of a compound and also its LD measured in rats can we use it when we design an experiment on mice ? (And vice versa)

08 September 2014 2,097 10 View

What would be the best animal model to study drug effects on Alzheimer's Disease?

If we have to select an animal to study either the effect of some drugs on Alzheimer's disease what would be the best choice ? Mice ? Rats ? Others ? Why ?

08 September 2014 2,220 4 View

Do you have protocols of primary cells cultures of hippocampal and cortical cells respectively (taken from monkey brain)?

See above

07 August 2014 939 1 View

What are the best choices for the cell culture to study Alzheimer's disease?

I plan to investigate the implications of a compound in Alzheimer's disease and conduct some pharmacological tests. What is the cell line（s） you suggest me to use?

07 August 2014 2,010 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View